Creatine Kinase (CK) Activity Assay Kit

Detection principle: Creatine kinase (CK, EC 2.7.3.2) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G6P-DH,Glucose-6-phosphate dehydrogenase) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm. Performance characteristics: Sample type: serum,plasma,animal tissue,cell Sensitivity: 6.16 U/L Detection range: 13.55-115.86 U/L Detection method: Colorimetric method Assay type: Enzyme Activity Assay time: 35 min Precision: Average inter-assay CV: 3.4-9.6%Average intra-assay CV: 2.5-7.4% Other reagents required: Double distilled water, PBS (0.01 mol/L, pH 7.4) Storage: 2-8? Valid period: 12 months. Protein function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N- phosphocreatine as heat without performing any mechanical or chemical work. [The UniProt Consortium]