Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

217 from 274 pages
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3CL Protease (T21I, S144A) (SARS-CoV-2) Assay Kit
3CL Protease (T21I, S144A) (SARS-CoV-2) Assay Kit

Item number: BPS-78834

The 3CL Protease (T21I, S144A) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of T21I, S144A mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. The 3CL Protease...
Keywords: main protease kit, Mpro, SARS-CoV-2 3CL Protease kit, untagged 3CL kit, 3 CL assay, 3CL Pro kit, nsp5,
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: SARS-CoV-2
1,983.00€ *
Review
3CL Protease (P252L) (SARS-CoV-2) Assay Kit
3CL Protease (P252L) (SARS-CoV-2) Assay Kit

Item number: BPS-78839

The 3CL Protease (P252L) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic based assay designed to measure the activity of P252L mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. 3CL inhibitor GC376 is...
Keywords: main protease kit, Mpro, SARS-CoV-2 3CL Protease kit, untagged 3CL kit, 3 CL assay, 3CL Pro kit, nsp5,
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: SARS-CoV-2
1,983.00€ *
Review
USP20 Inhibitor Screening Assay Kit
USP20 Inhibitor Screening Assay Kit

Item number: BPS-78840

The USP20 Inhibitor Screening Assay Kit is a 96-well format fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP20 for screening and profiling applications. To determine the effect of an inhibitor on USP20 activity, the enzyme should be preincubated with or without the test...
Keywords: hVDU2, USP20, KIAA1003, Ubiquitin thioesterase 20, Deubiquitinating enzyme 20, Ubiquitin carboxyl-terminal hydrolase 20,...
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: human
1,285.00€ *
Review
Amplite(R) Fluorimetric Sodium Ion Quantification Kit
Amplite(R) Fluorimetric Sodium Ion Quantification Kit

Item number: ABD-21325

Excitation (nm): 491 Emission (nm): 511. Quantifying sodium ions is important in various scientific fields and industries, including biochemistry, medicine, environmental analysis, and food science etc. There are several methods commonly used to quantify sodium ions, including flame photometry, ion-selective...
Application: Fluorimetric Na+ quantification
646.00€ *
Review
Amplite(R) Fluorimetric Lithium Ion Quantification Kit
Amplite(R) Fluorimetric Lithium Ion Quantification Kit

Item number: ABD-21351

Excitation (nm): 491 Emission (nm): 513. Quantifying lithium ions is important in various scientific fields and industries, including biochemistry, medicine, environmental analysis, and food science, etc. The rapid and accurate determination of lithium ions is particularly important in the battery industry. There...
Application: Fluorimetric Li+ quantification
646.00€ *
Review
Portelite(TM) Fluorimetric Lithium Ion Quantification Kit
Portelite(TM) Fluorimetric Lithium Ion Quantification Kit

Item number: ABD-21353

Excitation (nm): 491 Emission (nm): 513. Portelite(TM) Fluorimetric Lithium Ion Quantification Kit(TM) uses our robust lithium ion indicator dye, Lithiumighty(TM) 520, which exhibit great fluorescence intensity enhancement upon binding to Lithium ions. Lithiumighty(TM) 520 is the most robust lithium ion indicator...
Application: Fluorimetric Li+ quantification
646.00€ *
Review
SET7/9 Methyltransferase Inhibitor Screening Assay Kit
SET7/9 Methyltransferase Inhibitor Screening Assay Kit

Item number: Cay700270-96

SET7/9 is an MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. In Cayman's SET7/9 MT Inhibitor Screening Assay the transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates SAH, which is rapidly converted to...
Keywords: KMT7, SET Domain-containing Protein 7/9, SETD7/9
Application: Inhibitor screening
Species reactivity: human
1,146.00€ *
Review
SET8 Methyltransferase Inhibitor Screening Assay Kit
SET8 Methyltransferase Inhibitor Screening Assay Kit

Item number: Cay700350-96

SET Domain-containing Protein 8 (SET8) is a methyltransferase that selectively mono-methylates histone H4 at lysine residue 20 (H4K20), an event proven to have an important role in chromatin structure and transcriptional activation. Cayman's SET8 Methyltransferase Inhibitor Screening Assay provides a convenient...
Keywords: PR-Set7, SET domain-containing (lysine methyltransferase) 8, SETD8
Application: SET8 inhibitor screening
1,146.00€ *
Review
Caspase 2 Activity Assay Kit (Colorimetric Method)
Caspase 2 Activity Assay Kit (Colorimetric Method)

Item number: E-CK-A382.100

Caspase 2, also known as Ich-1 (ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis....
Keywords: Ich-1 (ICE and CED-3 homolog 1), Nedd-2, CASP2 activity, photometric assay
Application: Spectrophotometer, enzyme assay
Species reactivity: broad
From 69.00€ *
Review
Caspase 4 Activity Assay Kit (Colorimetric Method)
Caspase 4 Activity Assay Kit (Colorimetric Method)

Item number: E-CK-A384.100

Caspase 4, also known as Ich-2 (ICE and CED-3 homolog 2), also known as TX and ICErel-II, can interact with Apaf-1 and participate in caspase 3 activation caused by cytochrome c. Caspase 4 can also interact with caspase 14. This caspase 4 activity assay kit is based on caspase 4 that can catalyze the substrate...
Keywords: Ich-2 (ICE and CED-3 homolog 2), TX, ICErel-II, CASP4 activity, photometric assay
Application: Spectrophotometer, enzyme assay
Species reactivity: broad
From 69.00€ *
Review
Caspase 6 Activity Assay Kit (Colorimetric Method)
Caspase 6 Activity Assay Kit (Colorimetric Method)

Item number: E-CK-A386.100

Caspase 6, also known as Mch-2, was originally discovered in human Jurkat cells. The precursor of caspase 6 can be cleaved by granzyme B to form an activated caspase 6 dimer, which is found to induce apoptosis. Caspase 6 can cleave PARP and keratin-18, and also cleave Lamin A, a key component protein on the nuclear...
Keywords: Mch-2, CASP6 activity, photometric assay
Application: Spectrophotometer, enzyme assay
Species reactivity: broad
From 69.00€ *
Review
Caspase 8 Activity Assay Kit (Colorimetric Method)
Caspase 8 Activity Assay Kit (Colorimetric Method)

Item number: E-CK-A388.100

Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8...
Keywords: FLICE, MACH, Mch5, CASP8 activity, photometric assay
Application: Spectrophotometer, enzyme assay
Species reactivity: broad
From 69.00€ *
Review
217 from 274 pages