3CL Protease (P252L) (SARS-CoV-2) Assay Kit

The 3CL Protease (P252L) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic based assay designed to measure the activity of P252L mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic (FRET) peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS-79952).