Tumor Necrosis Factor Receptor Superfamily Member 1B, active (TNFRSF1B), Recombinant, Human

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. {ECO:0000269, PubMed:12370298}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.2ug/ml, corresponding to a specific activity of ? 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha. Sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT, Molecular Weight: 20.0kD, PubMed ID: 2172983, 2160731, 8661109, 14688072, 16710414, 15489334, 2173696, 8015639, 2153136, 1966549, 2166946, 11197692, 1328224, 8069916, 12370298, 25456591, 10206649, 11762942, 12161545, Gene Name: TNFRSF1B,TNFBR,TNFR2, Swiss Prot: P20333, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.