LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
Cay601170-96 96 wells -

5 - 10 Werktage*

83,00 €
Cay601170-480 480 wells -

5 - 10 Werktage*

310,00 €
 
Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability,... mehr
Produktinformationen "LDH Cytotoxicity Assay Kit"
Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced cytotoxicity. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman's LDH Cytotoxicity Assay Kit measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm. Synonyms: Lactate Dehydrogenase Cytotoxicity Assay Kit. Origin: Animal/Rabbit.
Schlagworte: Lactate Dehydrogenase Cytotoxicity Assay Kit
Hersteller: Cayman Chemical
Hersteller-Nr: 601170

Eigenschaften

Anwendung: Cell based assays
Reaktivität: Broad
Format: Homog.

Datenbank Information

KEGG ID : K00016 | Finde Alternativen

Handhabung & Sicherheit

Lagerung: -20°C
Versand: -20°C (International: -20°C)
Signalwort: Danger
GHS-Piktogramme:
H-Sätze: H227, H315, H316, H318, H319
P-Sätze: P210, P264, P280, P302+352, P305+351+338, P310, P321, P332+313, P337+313, P362+364, P370+378, P403, P501
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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