Zellviabilitäts- und Zytotoxizitätsassays

Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms. Autophagy is a multi-step process that begins...

Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced...

Assess changes in intracellular glutathione levels with the Intracellular GSH Assay Kit. This whole cell glutathione assay employs a proprietary thiol-sensitive dye, ThioBright(TM) Green, to monitor relative changes in the concentration of the free form of glutathione, aka GSH. Analyze the fluorescent signal by flow cytometry.

ICT's Intracellular Total ROS Activity Assay Kit provides a good screening option for assessing the potency of oxidative stress inhibitor and activator reagents, and will help to determine how oxidative stress modulates varied intracellular pathways. This kit assesses the overall level of intracellular ROS activity and can be analyzed using a flow cytometer.

A green CFSE membrane stain, is used to separate the effector cells from the target cells by staining all the target cells green. 7-AAD live/dead stain, measures cells killed by cytolytic activity by binding to the DNA of membrane-compromised cells.

A green CFSE membrane stain, is used to separate the effector cells from the target cells by staining all the target cells green. 7-AAD live/dead stain, measures cells killed by cytolytic activity by binding to the DNA of membrane-compromised cells.

Non radioactive single tube assay. Can further distinguish apoptosis from necrosis, leading to a more accurate assessment of cytolytic activity. Contains membrane stain, vital stain, and apoptosis reagent.

Non radioactive single tube assay. Can further distinguish apoptosis from necrosis, leading to a more accurate assessment of cytolytic activity. Contains membrane stain, vital stain, and apoptosis reagent.

This assay is based on the observation that blue and non-fluorescent resazurin is reduced to a pink fluorescent dye (resorufin) by accepting the electron from mitochondrial respiratory chain in live cells.

This kit uses a proprietary dye that gets enhanced fluorescence after esterase-catalyzed hydrolysis upon entering into live cells. Esterase activity is proportional to the number of vial cells.

This kit uses a proprietary dye that gets enhanced fluorescence after esterase-catalyzed hydrolysis upon entering into live cells. Esterase activity is proportional to the number of vial cells.

This particular kit uses two indicators- calcein AM for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. 96 wells: 100 assays, 394-wells: 400 assays.