Anti-RSK1 (Phospho-Thr359/Ser363) Rabbit Monoclonal Antibody (Clone RM233)

p90 ribosomal S6 kinase 1 (RSK1) is a member of the RSK family of serine/threonine kinases that mediates RAS/RAF/MEK/ERK/MAPK signaling. It contains N- and C-terminal kinase domains connected by a linker region, which contains various residues, including threonine 359 (Thr359) and serine 363 (Ser363), that are subject to phosphorylation and lead to its activation. RSK1 is ubiquitously expressed and localized to the cytoplasm, where it is in a complex with its activator ERK1/2 in quiescent cells. Upon stimulation of receptor tyrosine kinases (RTKs) by a variety of cytokines, neurotransmitters, or hormones, RSK1 accumulates at the plasma membrane and is phosphorylated by ERK1/2 and 3-phosphoinositide-dependent protein kinase 1 (PDK1), resulting in dissociation of the ERK1/2-RSK1 complex and activation of RSK1. RSK1 phosphorylates a variety of substrates that have roles in several cellular processes, including cell growth, survival, and proliferation. Tumor RSK1 levels are increased in patients with breast or prostate cancer. Cayman's RSK1 (Phospho-Thr359/Ser363) Rabbit Monoclonal Antibody (Clone RM233) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: p90 Ribosomal S6 Kinase 1 (phospho-T359/S363). Immunogen: Peptide corresponding to RSK1 (phospho-Thr359/Ser363). Clone Designation: RM233. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) RSK1 (phospho-Thr359/Ser363), (-) RSK1 without phosphorylation at Thr359/Ser363.