Anti-phospho-Vascular Endothelial Growth Factor Receptor 2 (Tyr1054, Tyr1059) (VEGF R2, Flk1, KDR, R

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine binding domain containing signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity. Applications: Suitable for use in Western blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilution to be determined by researcher. Positive Controls: Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF-1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR2. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.