Anti-phospho-p53 (Ser392) (Tumor Suppressor Protein, Oncogene Protein)

p53 is a multifunctional protein whose primary role is mediation of normal cell growth and suppression of tumor cell growth. Almost half of all human cancers show a defect in p53 function. p53 can induce apoptosis and cause cell cycle arrest. Phosphorylation of serine 392 has been demonstrated in vivo and in vitro by casein kinase II. The result of phosphorylation is site-specific DNA binding, strand renaturation, down-regulation of transcription and proliferation. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Positive Control: HEK 293 extracts, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.