Anti-phospho-Estrogen Receptor alpha, (Ser106) (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol R

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha. Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity. Applications: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:200, Western Blot: 1:500-1:1,000, Immunohistochemistry: 1:50-1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.