Anti-PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, MGC117256, Nicotinamide

Applications:, Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250-500, Immunohistochemistry (formalin-fixed paraffin): 1:250-500 (ABC, DAB), Direct ELISA: 1:3200-6400, Optimal dilutions to be determined by the researcher. Positive Control: Human visceral white fat tissue, rat and mouse liver tissue, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.