Anti-MUNC18-2 (Syntaxin-binding protein 2, Syntaxin-BP2, STXBP2, Hunc18b, Unc18-2)

MUNC18-2 (Syntaxin-binding protein 2, STXBP2, Unc18-2, Unc18B) is a 64-66kD member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2 and 3. Thus, it presumably participates in primary granule exocytosis by promoting granule fusion. Human STXBP2 is 593aa in length. It contains one SEC1 domain (aa28-576) with Trp28, Ser42 and Glu59 all playing a key role in syntaxin binding. There is one splice variant that shows a deletion of aa83-85. Over aa80-223, human and mouse STXBP2 share 95% amino acid sequence identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well., Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.