Anti-iNOS

Nitric Oxide Synthase (NOS) catalyzes the biosynthesis of nitric oxide from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS). iNOS is a soluble enzyme found in a variety of tissues including macrophages, hepatocytes, vascular smooth muscle cells, and chondrocytes. iNOS expression is increased by a variety of factors including LPS, IFN-gamma, Il-1beta, and TNF-alpha, whereas expression is deceased by dexamethasone. The enzyme has been cloned from several species including mouse, rat, and human with homology of at least 80% between these species. The calculated molecular weight of the protein from the deduced amino acid sequence is 130,000 - 131,000.Synonyms: Inducible Nitric Oxide Synthase, NOS II. Immunogen: Purified iNOS from cytokine-induced mouse macrophages (RAW 264.7) cells. Formulation: (Request formulation change), 500 µl of Protein A-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: IHC, IP, and WB. Cross Reactivity: (+) nNOS (5%), (-) eNOS. UniProt Accession #: P29477. Origin: Animal/Rabbit.