Anti-GABAA Receptor alpha5 Subunit

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS. They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: alpha1-6, beta1-4, gamma1-3, delta, epsilon, pi, theta, and rho1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by alpha1beta2gamma2 subunit-containing GABAA receptors, the major isoform found in the brain. The alpha subunit of GABAA receptors interfaces with a beta subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the gamma subunit. Approximately 5% of all GABAA receptors contain alpha5 subunits and up to 25% of these receptors are expressed in the hippocampus, a region of the brain associated with learning and memory. Deletions in GABRA5, which encodes the alpha5 subunit isoform, have been found on chromosome 15 in patients with Angelman syndrome who typically develop autism spectrum disorder (ASD). Levels of alpha5 subunit-containing GABAA receptors are decreased in postmortem hippocampal samples from patients with advanced Alzheimer's disease, Down syndrome, or fetal alcohol syndrome. Cayman's GABAA Receptor alpha5 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor alpha5 subunit at approximately 55 kDa from mouse and rat samples.Synonyms: gamma-Aminobutyric Acid Receptor Subunit alpha5, GABAA Receptor Subunit alpha5, GABRA5. Immunogen: Fusion protein from the cytoplasmic loop of the alpha5 subunit of the rat GABAA receptor. MW: ~55 kDa. Formulation: (Request formulation change), 100 µl of Affinity-purified rabbit polyclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC and WB.