Anti-COX-2 (Cyclooxygenase-2, PGHS 2, Prostaglandin Endoperoxide Synthase-2, PHS 2)

COX catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of COX by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothosis that the inducible form of COX, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.1,2 COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:300, Immunocytochemistry: 1:300, Western Blot: 1:200, Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.