Anti-CK1d (Casein Kinase 1 Delta, Casein kinase I Isoform Delta, CKI-delta, KC1D)

Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (a, a', b, y1-3, d, and e) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1e (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of b-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4). CK1d is involved in many cellular processes such as differentiation (5), cell growth and apoptosis (6-8), and control of the circadian rhythm (9). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.