Anti-CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eos

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul per 10e6 cells, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. After blocking, 10ul of C2099-92E was added to up to 1x10e6 cells and incubated for 30 minutes at RT., 3. Unbound antibody was removed by washing the cells twice in staining buffer (BSA-supplemented saline buffer). Note: that whole blood requires a RBC lysis step at this point., 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2a antibody. Absorption: 488nm , Emission: 565-605nm, Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.