Anti-Bax (BCL2-associated X protein, Apoptosis regulator BAX, cytoplasmic isoform beta, Apoptosis re

Bax is a 21kD protein that was identified as a protein that associates with Bcl-2, a repressor of apoptosis. Bax is a member of the bcl-2 family of proteins and contains Bcl-2 homology regions (BH-) 1, 2 and 3. Alternative splicing of Bax mRNA results in a 21kD membrane (alpha) and two forms of cytosolic proteins (beta and gama). Unlike Bcl-2, Bax initiates apoptosis when it homodimerizes or when it fails to form heterodimers with Bcl-2. Overexpression of Bax increased the rate of apoptosis caused by cytokine deprivation in an IL-3-dependent manner. Bax expression is stimulated by transcriptional activator p53, a tumor suppressor protein that induces apoptosis caused by damage to DNA. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot (ECL): 1:1000, Optimal dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb, I1904-46Q: IgG, H&L (HRP) Pab Gt xRb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.