0.5mg/ml if reconstituted with 0.2ml sterile DI water. Cytochrome p450, family 11, subfamily A, polypeptide 1, also called P450SCC, P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The...

Protein function: A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to...

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 37-526aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: TSTSSPRSFN EIPSPGDNGW LNLYHFWRES GTQKIHYHQM QSFQKYGPIY REKLGTLESV YIVDPKDASI LFSCEGPNPE RFLVPPWVAY...

Description: CYP11A1 Protein, Mouse, Recombinant (MBP & His-Avi), Biotinylated is expressed in E. coli with N-MBP, C-6xHis-Avi. The accession number is Q9QZ82.

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....