The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGM1. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Transglutaminase 1, Keratinocyte (TGM1) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGM1 in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.122ng/ml, Storage and Stability: Desiccation...

Accession Number: Q9JLF6/

Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, Immunohistochemistry: 5-20ug/ml, Immunocytochemistry: 5-20ug/ml, Optimal dilutions to be determined by the researcher. Included Protein...