Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Length: 2232 Sequence: atgggacata gagagattgt ggaacaccta ctggaccatg gagcagaagt aaatcatgag gatgttgatg gcaggactgc actctctgta gctgcacttt gtgtgcctgc aagtaaaggg cacgcatcag ttgttagcct tttaattgat cgaggtgctg aagtagatca ttgtgataaa gatggcatga ctccactgct ggtagctgcc tatgaaggac atgttgatgt ggttgacttg cttctagaag ggggagcaga...