Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Protein function: Acts as both a transcriptional activator and repressor (PubMed:29866902, PubMed:29848638). Binds to the DNA sequence 5'-ACAAT- 3' and shows a preference for guanine residues surrounding this core motif (PubMed:29866902). Binds to its own promoter and activates its own transcription...

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein acts as a transcriptional regulator when present in a complex with other proteins. It can activate p53...