Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

CD39L3 (CD39-like 3, Ectonucleoside Triphosphate Diphosphohydrolase-3, eNTPDase 3, HB6) is an 80kD, class I member of the GDA1/CD39 NTPase family of transmembrane (TM) enzymes. It is a cell-surface glycoprotein that hydrolyses both extracellular ATP and ADP. Thus, it controls ATP activation of P2X ion channel...