The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a2M. Next,...

Alpha-2-Macroglobulin (a2M) BioAssay(TM) ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of a2M in mouse serum, plasma, urine and other biological fluids. Detection Range: 3.70-300ng/ml, Sensitivity: 1.55ng/ml, Intra-Assay: CV<10%, Inter-Assay: CV<12%,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....