The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADAMTS7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADAMTS7. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Source:, Recombinant Rat from E. coli, Purity: >95%, Endotoxin: 1.0EU per 1ug (determined by the LAL method), Accession No: Q1EHB3, Fragment: Val444~Pro575 (Accession No: Q1EHB3), Sequence: MGHHHHHHSGSEF-VLPGVLY DVNHQCRLQY GPSSAYCEDV DNVCYTLWCS VGTTCHSKMD AAVDGTSCGK NKWCLNGECV PEGFQPETVD GGWSGWSAWS VCSRSCGVGV...

Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, Immunohistochemistry: 5-20ug/ml, Immunocytochemistry: 5-20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control...