The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GPX3. Next,...

Recognizes mouse GPX3. Detects a band of about 20-27kDa by Western blot. Weakly cross-reacts with human GPX3. Liquid. 0.2µm-filtered solution in PBS, pH 7.4. Contains no preservatives. Glutathione peroxidase 3 (GPX3, GSHPX3) is a selenium-dependent extracellular enzyme, protecting cells and enzymes from oxidative...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Sample Type:, Serum, plasma, tissue homogenates and other biological fluids, Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPX3 in mouse serum, plasma, tissue homogenates and other biological fluids. Sensitivity: 0.64ng/ml, Range: 1.56-100ng/ml, Specificity: This...