Rat GIPR/CRE Luciferase Reporter HEK293 Cell Line is an engineered HEK293 cell line expressing firefly luciferase under the control of cAMP response element (CRE), and rat GIPR (Rattus norvegicus Gastric Inhibitory Polypeptide receptor, NM_012714.2). Activation of GIPR in these cells can be monitored by measuring...

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 19-135aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: RAETDSEGQT TGELYQRWER YGWECQNTLE ATEPPSGLAC NGSFDMYACW NYTAANTTAR VSCPWYLPWY RQVAAGFVFR QCGSDGQWGS WRDHTQCENP EKNGAFQDQK LILERLQ. Purity:...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....