The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat 5HTR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 5HTR1A. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Serotonin Receptor 1A, also known as 5-hydroxytryptamine receptor 1A, is a member of the G protein-coupled receptor family. Serotonin Receptor 1A has been found to be expressed highly in the septum and hippocampus. Serotonin Receptor 1A seems to have an inhibitory effect on Ca2+ channels, via N-channels and...

ELISA Type: Sandwich ELISA, Double Antibody. Detection Range: 78.125-5000µg/mL. Sensitivity: 46.875µg/mL. Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples. Protein function: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor...