This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GnRH. Next, Avidin...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Gonadotropin Releasing Hormone (GnRH) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GnRH in mouse serum, plasma and other biological fluids. Detection Range: 12.35-1,000pg/ml, Sensitivity: 4.68pg/ml, Storage and Stability:...