The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HCII. Next,...

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT) (By similarity) Assay Type: Sandwich. Sensitivity: 0.12 ng/mL. Standard: 20 ng/mL. Detection range: 0.32-20 ng/mL....

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Sensitivity:, 4.688ng/ml, Range: 7.813-500ng/ml, Detection Method: Sandwich ELISA, Double Antibody, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing...