Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Ern1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering...

This affinity purified antibody is directed against mouse Ire1.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human. Protein function:...

This affinity purified antibody is directed against mouse Ire1.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human. Protein function:...