The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ASPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ASPN. Next,...

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 33-373aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: DMEDTDDDDN DDDDNSLFPT KEPVNPFFPF DLFPTCPFGC QCYSRVVHCS DLGLTSVPNN IPFDTRMVDL QNNKIKEIKE NDFKGLTSLY...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Asporin (ASPN) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ASPN in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.059ng/ml, Storage and Stability: Desiccation recommended. The Standard,...