For immunohistochemistry, flow cytometry, immunoprecipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

The Induced myeloid leukemia cell differentiation protein (MCL-1) TR-FRET Assay Kit (Rat) is designed to measure the inhibition of rat MCL-1 binding to its ligand in a homogeneous 384-well format. This TR-FRET based assay requires no time-consuming washing steps, making it especially suitable for high throughput...

Recombinant rat MCL1 (myeloid cell leukemia sequence 1) encompassing amino acids 2-307. This construct contains a N-terminal His-Tag (6xHis). The protein was affinity purified.

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....