Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16,000. , Western Blot: Preliminary experiments gave bands at approx 170kD and 26kD in Human Testis lysates after 1ug/ml antibody staining. Optimal dilutions to be determined by the researcher. Storage...

STAG3 is a meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates...