The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GaA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GaA. Next, Avidin...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

ELISA Type: Sandwich. Detection Range: 0.156-10µg/mL. Sensitivity: 0.094µg/mL. Sample Types: Serum, Plasma. Protein function: Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans. [The UniProt Consortium]