For flow cytometry to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

C5L2, also known as GPR77, is a 45kD 7TM receptor that binds the complement proteins , C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on , various hematopoietic cells where it promotes complement-mediated inflammation. ASP , binding to C5L2 on adipocytes triggers triglyceride...