The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BRAK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BRAK. Next,...

Organism: Rattus norvegicus (Rat). Source: E.Coli. Expression Region: 23-99aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE. Purity: >95% as determined by SDS-PAGE. Endotoxin: Less than...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Recombinant Rat BRAK, active (Cxcl14), Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10ng/ml. Sequence: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS MSRYRGQEHC LHPKLQSTKR...

ELISA Type: Sandwich. Detection Range: 93.75-6000µg/mL. Sensitivity: 56.25µg/mL. Sample Types: Serum, plasma and other biological fluids