Description: gp130/IL6ST Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 68.2 kDa and the accession number is H9BFG4.

Description: gp130/IL6ST Protein, Rat, Recombinant (His & mFc) is expressed in HEK293 mammalian cells with His, mFc tag. The predicted molecular weight is 94.3 kDa and the accession number is H9BFG4.

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Glycoprotein 130 is a ubiquitously expressed, 130kD type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane. Rat gp130 is synthesized as a 918aa precursor with a 22aa signal...

Activity: Measured by its ability to inhibit the IL6 Ra enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.5-2 µg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6. Protein...