Secretin is a neuropeptide hormone that regulates secretion from the stomach, pancreas, and liver. It binds to the rat secretin receptor (SCT-R, Ki = 3.3 nM) and increases intracellular cAMP (EC50 = 1 nM) in COS cells expressing rat SCT-R. It also increases SCT-R phosphorylation 7.2-fold at a concentration of 0.1...

Sample Types: serum, plasma, tissue homogenates Detection Range: 3.12 pg/mL-200 pg/mL Sensitivity: 0.78 pg/mL Assay Principle: quantitative Measurement: SandwichAssay Time: 1-5h Sample Volume: 50-100ulDetection Wavelength: 450 nmProtein function: Hormone involved in different processes, such as regulation of the pH...

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCT. Next, Avidin conjugated...