The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next,...

Plasminogen activator inhibitor-2 (placental PAI) is a coagulation factor that inactivates tPA and urokinase. It is present in most cells, especiallymonocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Plasminogen Activator Inhibitor 2 (PAI2) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAI2 in mouse plasma. Detection Range: 78-5000pg/ml, Sensitivity: 34pg/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Kit Components: *027476A: Microtiter Strip, 1x96...