Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family. Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non-collagenous regions....