O6-methylguanine-DNA Methyltransferase (MGMT) is a DNA repair enzyme responsible for demethylating O6-methylguanine (O6-mG). The methylation of guanine at O6 to form O6-mG allows base-pairing to thymine rather than cytosine during DNA replication. MGMT prevents mutations by transferring the methyl group from O6-mG...

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 4-238aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: QPAPLERFAS RRPQVLAVRT VCDLVLGKMD KDCEMKRTTL DSPLGKLELS GCEQGLHEIK LLGKGTSAAD AVEVPAPAAV LGGPEPLMQC TAWLNAYFHQ PEAIEEFPVP ALHHPVFQQE SFTRQVLWKL LKVVKFGEVI...

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1:...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....