Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Length: 1704 Sequence: atggaagcgg agaggcggcc ggcgccgggc tcgccctccg agggcctgtt tgcggacggg cacctgatct tgtggacgct gtgctcggtc ctgctgccgg tgttcatcac cttctggtgt agcctccagc ggtcgcgccg gcagctgcac cgcagggaca tcttccgcaa gagcaagcac gggtggcgcg acacggacct gttcagccag cccacctact gctgcgtgtg cgcgcagcac attctgcagg gcgccttctg...