Description: BHQ-1 NHS is a compound that is directly labeled with fluorescently labeled nucleotide. Smiles: O=C(CCCN(C)C1=CC=C(C=C1)N=NC2=CC(C)=C(C=C2OC)N=NC3=CC=C(C)C=C3[N+]([O-])=O)ON4C(CCC4=O)=O

UGQuencher-1 NHS ester, which is functionally equivalent to BHQ 1 NHS ester, is a dark quencher with a polyaromatic-azo backbone that ensures no intrinsic fluorescence. Its broad and intense absorption range (480-580 nm) makes it highly effective for quenching in fluorescence-based assays, particularly in qPCR...