Description: Dimethylenastron, an Eg5 inhibitor, arrests cells with monopolar spindles to which all chromosomes attach in a syntelic manner. Target: Kinesin. Smiles: O=C1C=2C(NC(=S)NC2CC(C)(C)C1)C3=CC(O)=CC=C3. References: Aljaberi AM, et al. Cell Cycle. 2015,14(11):1738-47.

Dimethylenastron is an inhibitor of the mitotic kinesin Eg5 (IC50 = 200 nM in an ATPase assay). It is selective for Eg5 over other kinesin subfamilies from four different organisms. Dimethylenastron induces accumulation of HeLa cells in the G2/M phase (75% at a concentration of 1 µM). It inhibits spindle formation...