Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be...

Tricine is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...