The 5-isomer of fluorescein dichlorotriazine (5-DTAF) is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and with hydroxy groups.

Description: 5-DTAF, an isomeric derivative of fluorescein, reacts directly with polysaccharides and alcohols in aqueous solutions at pH > 9. Its emission profile peaks at a wavelength of 518 nm following excitation at 488 nm [1]. Target: Others. Smiles: Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(Nc4nc(Cl)nc(Cl)n4)ccc23)c1