Upon interaction with phosphatases the colorless and non-fluorescent FDP is hydrolyzed to highly fluorescent fluorescein. FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases.

Absorbance (nm): 487 Correction Factor (260 nm): 0.32 Correction Factor (280 nm): 0.35 Extinction coefficient (cm^-1 M^-1): 80000 (0.1N NaOH) Excitation (nm): 498 Emission (nm): 517 Quantum yield: 0.7900 (EtOH (neutral)), 0.95 (0.1N NaOH). Upon interaction with phosphatases the colorless and non-fluorescent FDP is...

Description: Fluorescein diphosphate tetraammonium salt is a colorless and nonfluorescent substrate for alkaline phosphatases. The sequential alkaline phosphatase mediated hydrolysis of its two phosphate substituents yielding weakly fluorescent fluorescein monophosphate followed by strongly fluorescent fluorescein....