Dansyl amide is a fluorescent probe for albumin and CAII. It binds to serum albumin and carbonic anhydrase II (CAII, Kds = 7.57 and 0.2 µM, respectively) and has been used to determine ligand affinities for these proteins. Dansyl amide binding to CAII results in an increase in fluorescence due to FRET from nearby...

Dansylamide (DNSA) is a fluorescent dye that forms in a reaction between dansyl chloride and ammonia. Dansylamide is widely used in biochemistry and chemistry to label substances with the fluorescent dansyl group. The dansyl group can be introduced into amino acids or other amines. The excitation maximum (350 nm) is...