T7 mRNA Transcription Kit (Cap-1, 5mCTP, PsiUTP)

Cayman's T7 mRNA Transcription Kit (Cap-1, 5mCTP, psiUTP) is optimized for the efficient synthesis of high-quality mRNA from a linear DNA template containing a T7 promoter sequence. Using T7 RNA polymerase, this kit enables the production of mRNA with high yield in vitro, which is suitable for a variety of downstream applications. The kit includes all essential reagents, including Cap-1, a modified 5'-cap analog, 5-methylcytidine-5'-triphosphate (5mCTP), and psiUTP. The cap facilitates efficient translation initiation, protects against exonuclease-mediated degradation, and improves the stability of the mRNA when delivered via lipid nanoparticles (LNPs) or other formulations. 5mCTP and psiUTP reduce host cell immune response and also enhance mRNA stability. Each kit contains sufficient reagents for 25 reactions (20 µl per reaction). Each reaction can yield up to 100 µg of RNA.. Formulation: . InChI: . InChIKey: . SMILES: